Sanger sequencing, also known as chain-termination sequencing or dideoxy sequencing, has been the driving force of DNA sequencing since its inception in the 1970s and is still considered a gold standard method that relies on the detection of tagged chain-terminating nucleotides inserted by a DNA polymerase during iso-thermal template replication.

Routine validation of cloning studies, PCR fragments, Functional and comparative genomics, evolutionary genetics, and complicated disease research have all benefited from the technique.

DBT supported Genomic DNA sequencing facility that started in 1997 houses 16-,96- capillary Sanger sequencers, AB 9700 PCR machines. This national facility houses state of the art instrumentation and serves national R&D and academic establishments having DSIR authorization and Customs exemption such as colleges, public labs, government foundations, and not-for-benefits associations.

More than 100 users across India are exploiting the services offered by this facility. Users from distant parts of the country send their samples through the mail and others give their samples by hand in the form of gDNA, live E. coli cultures, plasmids, or PCR products. The trained and experienced staff processes and analyzes the samples for their integrity and quality of samples by gel electrophoreses. After labeling with fluorescently labeled chain-terminating dideoxy-nucleotides in iso-thermal replication reaction, the samples are run through a capillary system that reads the fluorescence and gives the output in the form of an electropherogram.  The Machines can read up to 16-/96- samples simultaneously, increasing throughput manifold and making the workflow time saving and economical.

The facility has staff with expertise in primer walking to sequence long DNA sequences and complete plasmids.

Institutions from all over the country have availed services from this facility.

Sample submission instructions and guidelines