Sanger Sequencing

Sanger sequencing, also known as chain-termination sequencing or dideoxy sequencing, has been the driving force of DNA sequencing since its inception in the 1970s and is still considered a gold standard method that relies on the detection of tagged chain-terminating nucleotides inserted by a DNA polymerase during iso-thermal template replication.

Routine validation of cloning studies, PCR fragments, Functional and comparative genomics, evolutionary genetics, and complicated disease research have all benefited from the technique.

DBT supported Genomic DNA sequencing facility that started in 1997 houses 16-,96- capillary Sanger sequencers, AB 9700 PCR machines. This national facility houses state of the art instrumentation and serves national R&D and academic establishments having DSIR authorization and Customs exemption such as colleges, public labs, government foundations, and not-for-benefits associations.

More than 100 users across India are exploiting the services offered by this facility. Users from distant parts of the country send their samples through the mail and others give their samples by hand in the form of gDNA, live E. coli cultures, plasmids, or PCR products. The trained and experienced staff processes and analyzes the samples for their integrity and quality of samples by gel electrophoreses. After labeling with fluorescently labeled chain-terminating dideoxy-nucleotides in iso-thermal replication reaction, the samples are run through a capillary system that reads the fluorescence and gives the output in the form of an electropherogram.  The Machines can read up to 16-/96- samples simultaneously, increasing throughput manifold and making the workflow time saving and economical.

The facility has staff with expertise in primer walking to sequence long DNA sequences and complete plasmids.

Institutions from all over the country have availed services from this facility.

Sample submission instructions and guidelines

List of institutions who have used the facility services

S.No. Institute State Numbers of samples
1 New Delhi AIIMS
2 New Delhi Department of Biochemistry, University of Delhi
3 New Delhi Department of Botany, University of Delhi
4 New Delhi Department of Genetics University Of Delhi
5 New Delhi Department of Microbiology, University of Delhi
6 New Delhi Department of Plant Molecular Biology, University of Delhi
7 New Delhi Department of Zoology, University of Delhi
8 New Delhi Deshbandhu Collge
9 New Delhi ICAR-Nagtional Research Centre for Plant Biotechnology
10 New Delhi ICGEB
11 New Delhi IIT Delhi
12 New Delhi Indian Agriculture Research Institute
13 New Delhi Institute of Liver & Biliary Sciences
14 New Delhi JNU
15 New Delhi National Institue of Immunology
16 New Delhi Ramjas College
17 New Delhi Safdarjung Hospital
18 New Delhi Sir Ganga Ram Hospital
19 New Delhi Sri Venkateshwara College
20 New Delhi The Energy Resource Institute
21 West Bengal Bose Institute, Kolkata
22 West Bengal IVRI Kolkata
23 West Bengal National Institue of Technology, Durgapur
24 West Bengal West Bengal University
25 Uttar Pradesh Amity University , Noida
26 Uttar Pradesh Central Drug Research Institute, Lucknow
27 Uttar Pradesh Central Food Technology Research Institute, Lucknow
28 Uttar Pradesh Indian Vegitable Research Institute, Varanasi
29 Uttar Pradesh IVRI Bareilly
30 Uttar Pradesh IVRI Varanasi
31 Uttar Pradesh Jaypee Institute of Technology, Noida
32 Uttar Pradesh National Beuro of Fish Genetic Resources, Lucknow
33 Uttar Pradesh National Botanical Research Institute, Lucknow
34 Uttar Pradesh SG Post Graduate Institute of Medical Science, Lucknow
35 Punjab GADVASU Ludhiana
36 Punjab IMTECH Chandigarh
37 Punjab Institute of Microbial Technology, Chandigarh
38 Punjab Patiala University
39 Punjab SG Post Graduate Institute of Medical Science, Chandigarh
40 Punjab Thapar University, Patiala
41 Hariyana Maharshi Dayanand University, Rohtak
42 Hariyana National Brain Research Institute, Hisar
43 Hariyana National Research Centre for Camel, Hisar
44 Hariyana National Research Centre for Equine, Hisar
45 Hariyana Reginall Centre for Biotechnology, Faridabad
46 Rajasthan BITS Pilani
47 Rajasthan Cental Arid Zone Research Institute, Bikaner
48 Rajasthan National Research Centre for Camel, Bikaner
49 Rajasthan Rajasthan University of Venterinary & Animal Sciences, Bikaner
50 Maharastra National Centre for Cell Sciences, Pune
51 Maharastra National Environmental Engineering Research Institute, Pune
52 Maharastra Serum Institute of India, Pune
53 Assam IIT Guwahati
54 Oddisa IIT Bhubneshwar
55 Uttarakhand G B Pant Agriculture Univrsity & Technology, Pantnagar
56 Uttarakhand IVRI Mukteshwara
57 Madhya Pradesh IVRI Bhopal
58 Himachal Pradesh National Research Centre for Mushroom, Palampur
59 Arunachal Pradesh National Research Centre for Mithun, Dirang
60 Nagaland National Research Centre for Yalk
61 Mizoram Central Agriculture University
62 Mizoram Mizoram University
63 Tamilnadu Sugarcane Breeding Institute, Coimbatore
64 Karnataka IISc Bangalore
65 Jammu Jammu University
66 Chhattisgarh Central Institue of Biotic Stress Management, Raipur
67 Kerala Rajiv Gandhi Centre for Biotechnology, Trivandrum

Sample submission forms and pricing details

Regular DNA Sequencing Services
Service code Item Total cost per sample
A1 Sequencing of Cloned DNA and PCR products up to 650* bases using standard primers (T7, T3, SP6, M13R,M13F) or with user supplied primers. Rs. 650/-
B1 Up to 650 bases*: (primer will be synthesized by the facility) Rs. 1650/-
C0 Upto 1.0 kb single stranded sequencing Rs. 1800/-
Single Stranded Primer Walking Sequencing
Service code Item Total cost per sample
C1 Upto 1.5 kb single stranded sequencing Rs. 2950/-
C2 Upto 2.0 kb single stranded sequencing Rs. 4600/-
C3 Upto 2.4 kb single stranded sequencing Rs. 5600/-
C4 Upto 3.0 kb single stranded sequencing Rs. 7900/-
C5 Upto 3.6 kb single stranded sequencing Rs. 8900/-
C6 Upto 4.2 kb single stranded sequencing Rs. 11200/-
C7 Upto 4.8 kb single stranded sequencing Rs. 13500/-
Double Stranded Primer Walking Sequencing
Service code Item Total cost per sample
D1 Upto 1.0 kb double stranded sequencing Rs. 4600/-
D2 Upto 1.5 kb double stranded sequencing Rs. 5900/-
D3 Upto 2.0 kb double stranded sequencing Rs. 11200/-
D4 Upto 2.5 kb double stranded sequencing Rs. 14500/-
D5 Upto 3.0 kb double stranded sequencing Rs. 17800/-
D6 Upto 3.5 kb double stranded sequencing Rs. 19450/-
D7 Upto 4.0 kb double stranded sequencing Rs. 21100/-